目的 研究标准望春花油的急性毒性和抗炎作用。方法 采用5种动物模型研究标准望春花油的抗炎作用,包括二甲苯致小鼠耳肿胀,角叉菜胶致大鼠足肿胀,组胺致大鼠毛细管通透性增加,棉球诱导大鼠肉芽肿的形成,卵蛋白致豚鼠鼻黏膜免疫性炎症等。采用急性口服毒性实验研究标准望春花油的急性毒性。结果 口服标准望春花油能明显抑制二甲苯致小鼠耳肿胀,角叉菜胶致大鼠足肿胀,组胺致大鼠毛细管通透性增加,棉球诱导大鼠肉芽肿的形成及卵蛋白致豚鼠鼻黏膜毛细血管通透性增加,并且表现出显著的剂量依赖性抗炎作用。此外,连续7 d给小鼠以2.5 g·kg-1剂量灌胃给药,未出现明显的急性毒性。结论 标准望春花油具有很好的抗炎作用且无明显的急性毒性,其作用机制可能与其多靶点抑制炎性介质及炎性增生有关。
Abstract
Objective To investigate the anti-inflammatory effect and acute toxicity of standard Magnolia biondii Pamp oil(SMBO). METHODS The anti-inflammatory effect of SMBO was evaluated in five experimental animal models,including xylene-induced mice ear edema,carrageenin-induced rat hind paw edema,histamine-induced rat vascular permeability,cotton pellet-induced rat granuloma formation,and guinea pig schneiderian membrane immuno-inflammation. In addition,the 7-day acute oral toxicity of SMBO was evaluated. RESULTS Pretreatment with a single dose of SMBO produced significant dose-dependent anti-inflammatory effects on xylene-induced mice ear edema,carrageenin-induced rat hind paw edema,histamine-induced rat vascular permeability,cotton pellet-induced rat granuloma formation,and guinea pig schneiderian membrane immuno-inflammation. In addition,a single oral dose of SMBO at 2.5 g穔g-1 body weight produced no observable acute toxicity in mice within seven days. CONCLUSION SMBO possesses potent anti-inflammatory effect and has no observable acute oral toxicity,and the mechanism may be related with the inhibition of multi- inflammatory mediators and inflammatory hyperplasia.
关键词
标准望春花油 /
抗炎作用 /
急性毒性
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Key words
standard Magnolia biondii Pamp oil /
anti-inflammatory effect /
acute toxicity
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中图分类号：
R905
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参考文献
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脚注
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基金
国家973计划资助项目（2006CB504707）
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